For non-protein thiol (NPTs) content, the fresh plant sample was extracted with 5% sulfosalicylic acid and the supernatant was saved form 10,000 g , 15 min, 4 C
Qualitative assays are simple and less expensive, so these assays are applied for screening newborns in some nations
They have a defensive role against potato blight ( Phytophthora infestans ) especially, and they are found both in free and esterified states and are toxic to many other plant pathogens
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Ethics declarations Competing interests RS and JPR received research funding for this study from Researched Nutritionals, LLC
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